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KMID : 0816119990020020185
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Korean Journal of Pediatric Gastroenterolology and Nutrition
1999 Volume.2 No. 2 p.185 ~ p.193
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Ultrastructural and Immunohistochemical Study of Hepatic Fibrosis after the Ligation of the Common Bile Duct in Rats
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Moon Kyung-Rye
Park Sang-Kee
Rho Young-Il
Park Yeong-Bong
Suh Chae-Hong
Kim Man-Woo
Seo Woo-Chul
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Abstract
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Purpose : Proliferation of bile duct-like structures and fibrosis is a hepatic cellular
reaction observed in most forms of human liver disease and in a variety of experimental
conditions associated with liver injury. The aim of this study was to investigate the
activation of Ito cells and bile duct proliferation in the rat after common bile duct
ligation (CBDL).
Methods : Hepatic morphological abnormalities were examined in rats whose bile ducts
had been irreversibly ligated for 15, 21, 24 and 28 days. The liver was examined by
immunohistochemical staining for α-smooth muscle actin, the blown marker of activated
Ito cells, and light and electron microscopes.
Results : After CBDL, the bile canalicular proliferation and interstitial fibrosis were
gradually increased in the periportal areas extended to hepatic sinusoids. Ito cells
positive for α-smooth muscle actin were frequently observed in the periductular space
and in perisinusoidal space of Disse. Ito cells and myofibroblasts were gradually
increased in the interstitial fibrosis until the 28th day after CBDL. Ito cells and
myofibroblasts had microfilaments with dense body at the periphery of the cell.
Conclusions : Our results suggest that Ito cells may be fibroblastic or myogenic. It
has also been postulated that during the development of hepatic fibrosis, Ito cells
become myofibroblasts or fibroblast like cells.
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KEYWORD
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Hepatic fibrosis, Ito cell, Ligation of common bile duct,
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